FeCo-Chitosan / DNA nanoparticles for gene transfer to MCF-7 breast cancer cells: preparation and characterization
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Abstract
Background: Researchers have seen gene therapy as one of the most important techniques for treating illnesses including cancer and a range of genetic problems in recent years. The capacity of FeCo-Chitosan nanoparticles for gene transport into MCF-7 cells was explored in this study.Methods: FeCo-Chitosan/DNA nanoparticles were prepared. Then, the physicochemical features of nanoparticles were assessed using SEM. Also, biological features of the nanoparticles including biocompatibility, DNA protection, DNA release, and gene transfer capacity to MCF-7 cells were studied.Results: The Results showed that FeCo-Chitosan / DNA nanoparticles exhibited a spherical shape with an average size of around 200 nm. The zeta potential of the FeCo-Chitosan/DNA complex increased with increasing the concentration of FeCo-Chitosan nanoparticles in the FeCo-Chitosan/DNA complex. Electrophoretic analyses showed that FeCo-Chitosan/DNA nanoparticles protect DNA against nuclease degradation and ultrasonic damage. Also, the MTT test revealed that FeCo-Chitosan nanoparticles had a good biocompatibility.Conclusions: FeCo-Chitosan nanoparticles may safely transfer and release DNA to MCF-7 cells, according to fluorescence microscopy and flow cytometry studies. These findings also revealed that increasing the concentration of FeCo-Chitosan in the FeCo-Chitosan/DNA complex improved gene transfer efficiency
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28.Amani A, Zare N, Asadi A, ZAKARIA RA. Ultrasound-enhanced gene delivery to alfalfa cells by hPAMAM dendrimer nanoparticles. Turkish Journal of Biology. 2018;42(1):63-75.
2.Tang S, Huang Z, Zhang H, Wang Y, Hu Q, Jiang H. Design and formulation of trimethylated chi¬tosan-graft-poly (ɛ-caprolactone) nanoparticles used for gene delivery. Carbohydrate polymers. 2014;101:104-12.
3.Zwicke GL, Ali Mansoori G, Jeffery CJ. Utilizing the folate receptor for active targeting of cancer nanoth¬erapeutics. Nano reviews. 2012;3(1):18496.
4.Zintchenko A, Philipp A, Dehshahri A, Wagner E. Simple modifications of branched PEI lead to highly efficient siRNA carriers with low toxicity. Bioconju¬ chemistry. 2008;19(7):1448-55.
5.Nikolova MP, Chavali MS. Metal oxide nanoparticles as biomedical materials. Biomimetics. 2020;5(2):27.
6.Li HL, He YX, Gao QH, Wu GZ. Folate polyethyl¬ene glycol conjugated carboxymethyl chitosan for tumor targeted delivery of 5 fluorouracil. Molecular medicine reports. 2014;9(3):786-92.
7.Hashemi M. siRNA delivery improvement by co-for¬mulation of different modified polymers in eryth¬roleukemic cell line K562. Iranian journal of basic medical sciences. 2013;16(9):973.
8.Hu F, Chen W, Zhao M, Yuan H, Du Y. Effective antitumor gene therapy delivered by polyethylen¬imine-conjugated stearic acid-g-chitosan oligosac¬charide micelles. Gene Therapy. 2013;20(6):597-606.
9.Bentley-Goode KA, Newton NJ, Thompson AM. Business strategy, internal control over financial reporting, and audit reporting quality. Auditing: A Journal of Practice & Theory. 2017;36(4):49-69.
10. Fuchigami T, Kawamura R, Kitamoto Y, Nakagawa M, Namiki Y. A magnetically guided anti-cancer drug delivery system using porous FePt capsules. Bi-omaterials. 2012;33(5):1682-7.
11. Abdolmaleki A, Asadi A, Ardabili M, Namin I. Impor¬tance of Nano Medicine and New Drug Therapies for Cancer. Advanced Pharmaceutical Bulletin. 2020.
12. Sherlock SP, Dai H. Multifunctional FeCo-graphit¬ic carbon nanocrystals for combined imaging, drug delivery and tumor-specific photothermal therapy in mice. Nano Research. 2011;4(12):1248-60.
13. Kuchi R, Lee K-M, Lee Y, Luong CH, Lee K-D, Park B-G, et al. Synthesis of highly magnetic FeCo nano¬particles through a one pot polyol process using all metal chlorides precursors with precise composition tunability. Nanoscience and Nanotechnology Letters. 2015;7(9):734-7.
14.Hu J, Zhu M, Liu K, Fan H, Zhao W, Mao Y, et al. A biodegradable polyethylenimine-based vector modified by trifunctional peptide R18 for enhanc¬ing gene transfection efficiency in vivo. PLoS One. 2016;11(12):e0166673.
15.Chen J, Tian B, Yin X, Zhang Y, Hu D, Hu Z, et al. Preparation, characterization and transfection ef¬ficiency of cationic PEGylated PLA nanoparticles as gene delivery systems. Journal of Biotechnology. 2007;130(2):107-13.
16.Kichler A, Leborgne C, Coeytaux E, Danos O. Poly¬ethylenimine‐mediated gene delivery: a mechanistic study. The journal of gene medicine. 2001;3(2):135-44.
17.Kulkarni JA, Myhre JL, Chen S, Tam YYC, Danescu A, Richman JM, et al. Design of lipid nanoparticles for in vitro and in vivo delivery of plasmid DNA. Na-nomedicine: Nanotechnology, Biology and Medicine. 2017;13(4):1377-87.
18.Ashfaq UA, Riaz M, Yasmeen E, Yousaf MZ. Recent advances in nanoparticle-based targeted drug-de¬livery systems against cancer and role of tumor mi-croenvironment. Critical Reviews™ in Therapeutic Drug Carrier Systems. 2017;34(4).
19.Wang Z, Yu L, Ding M, Tan H, Li J, Fu Q. Preparation and rapid degradation of nontoxic biodegradable pol¬yurethanes based on poly (lactic acid)-poly (ethylene glycol)-poly (lactic acid) and l-lysine diisocyanate. Polymer Chemistry. 2011;2(3):601-7.
20.Li L, Gao F, Jiang W, Wu X, Cai Y, Tang J, et al. Folic acid-conjugated superparamagnetic iron oxide nano¬particles for tumor-targeting MR imaging. Drug de-livery. 2016;23(5):1726-33.
21.Lee J-L, Lo C-W, Inserra C, Béra J-C, Chen W-S. Ultrasound enhanced PEI-mediated gene delivery through increasing the intracellular calcium level and PKC-δ protein expression. Pharmaceutical re¬search. 2014;31(9):2354-66.
22.Bauhuber S, Liebl R, Tomasetti L, Rachel R, Goep¬ferich A, Breunig M. A library of strictly linear poly (ethylene glycol)–poly (ethylene imine) diblock co-polymers to perform structure–function relationship of non-viral gene carriers. Journal of controlled re¬lease. 2012;162(2):446-55.
23.Eggenberger K, Frey N, Zienicke B, Siebenbrock J, Schunck T, Fischer R, et al. Use of nanoparticles to study and manipulate plant cells. Advanced Engi-neering Materials. 2010;12(9):B406-B12.
24.He C, Klionsky DJ. Regulation mechanisms and sig¬ naling pathways of autophagy. Annual review of ge¬netics. 2009;43:67-93.
25.Lee S, Son SJ, Song SJ, Ha TH, Choi JS. Polyamidoam¬ine (PAMAM) dendrimers modified with cathep¬sin-B cleavable oligopeptides for enhanced gene de-livery. Polymers. 2017;9(6):224.
26.Yang F, Yao J, Min J, Li J, Chen X. Synthesis of high saturation magnetization FeCo nanoparticles by polyol reduction method. Chemical Physics Letters. 2016;648:143-6.
27.Ge C, Du J, Zhao L, Wang L, Liu Y, Li D, et al. Binding of blood proteins to carbon nanotubes reduces cytotoxicity. Proceedings of the National Academy of Sciences. 2011;108(41):16968-73.
28.Amani A, Zare N, Asadi A, ZAKARIA RA. Ultrasound-enhanced gene delivery to alfalfa cells by hPAMAM dendrimer nanoparticles. Turkish Journal of Biology. 2018;42(1):63-75.
| Files | ||
| Issue | Vol 13 No 4 (2021) | |
| Section | Original Articles | |
| DOI | https://doi.org/10.18502/bccr.v13i4.14399 | |
| Keywords | ||
| Chitosan FeCo Gene delivery Magnetic properties Cancer Nanopar | ||
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How to Cite
1.
Abdolmaleki A, Omar Khudhur Z, Wasman Smail S, Asadi A, Amani A. FeCo-Chitosan / DNA nanoparticles for gene transfer to MCF-7 breast cancer cells: preparation and characterization. Basic Clin Cancer Res. 2022;13(4):245-257.
