Tehran University of Medical Sciences Basic & Clinical Cancer Research 2228-6527 13 4 2022 10 24 245 257 EN Arash Abdolmaleki Department of Bioinformatics, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran Zhikal Omar Khudhur Department of Medical Analysis, Faculty of Applied Science, Tishk International University - Erbil, Kurdistan Region, Iraq Shukur Wasman Smail Department of Biology, College of Science, Salahaddin University-Erbil, Iraq AND Department of Biology, College of Science, Cihan University-Erbil, Kurdistan Region, Iraq Asadollah Asadi Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran. Amin Amani Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabil, Iran 2022 02 15 2022 05 31 Background: Researchers have seen gene therapy as one of the most important techniques for treating illnesses including cancer and a range of genetic problems in recent years. The capacity of FeCo-Chitosan nanoparticles for gene transport into MCF-7 cells was explored in this study. Methods: FeCo-Chitosan/DNA nanoparticles were prepared. Then, the physicochemical features of nanoparticles were assessed using SEM. Also, biological features of the nanoparticles including biocompatibility, DNA protection, DNA release, and gene transfer capacity to MCF-7 cells were studied. Results: The Results showed that FeCo-Chitosan / DNA nanoparticles exhibited a spherical shape with an average size of around 200 nm. The zeta potential of the FeCo-Chitosan/DNA complex increased with increasing the concentration of FeCo-Chitosan nanoparticles in the FeCo-Chitosan/DNA complex. Electrophoretic analyses showed that FeCo-Chitosan/DNA nanoparticles protect DNA against nuclease degradation and ultrasonic damage. Also, the MTT test revealed that FeCo-Chitosan nanoparticles had a good biocompatibility. Conclusions: FeCo-Chitosan nanoparticles may safely transfer and release DNA to MCF-7 cells, according to fluorescence microscopy and flow cytometry studies. These findings also revealed that increasing the concentration of FeCo-Chitosan in the FeCo-Chitosan/DNA complex improved gene transfer efficiency https://bccr.tums.ac.ir/index.php/bccrj/article/view/415