Mouse spontaneous mammary adenocarcinoma as a suitable model of breast cancer in Iran

Abstract

Background: Breast cancer is a complicated disease and has various clinical manifestations and prognosis. The aim of this study is to make a breast cancer model suitable for basic, preclinical and clinical studies.Materials and methods: Five virgin Bulb/c mice aged 6 weeks and spontaneous Murine Mammary Adenocarcinoma Cells (MMAC) were used. After anesthesia induction, 1-3 million of MMACs were subcutaneously injected into the mice’s right flank. Four weeks after the tumor induction, tumor size in both length and width were weekly measured using caliper for 4 weeks. Histopathology studies were done 2 months later by Scraff- Boom-Richardson grading.Results: Visible tumors were appeared after 4 weeks. Take-rate of all cells was nearly 100%. Tumor sizes reached 0.6±0.1 mm3, 1.1±0.3, 1.68±0.5 and 3±0.7 cm3 with at fifth, sixth, seventh and eighth weeks respectively. The histopathological studies using H & E method showed invasive ductal carcinoma, grade II/III.Conclusions: Elucidation of the molecular mechanisms of breast cancer progression and metastasis has extremely gained from mouse models in which some stages of tumor progression are recapitulated.