Tehran University of Medical Sciences Basic & Clinical Cancer Research 2228-6527 3 3&4 2012 09 29 30 33 EN Ali Mohammad Alizadeh Cancer Research Center, Tehran University of Medical Science, Tehran, I.R. Iran Saleh Azizian Cancer Research Center, Tehran University of Medical Science, Tehran, I.R. Iran Hamid Reza Fayazi Azad Islamic University-Varamin Pishvar Branch, Varamin, I.R. Iran Maryam-Sadat Mousavi Cancer Research Center, Tehran University of Medical Science, Tehran, I.R. Iran Seyed Ali Nabavi Azad Islamic University-Varamin Pishvar Branch, Varamin, I.R. Iran 2012 09 29 2012 09 29 Background: Breast cancer is a complicated disease and has various clinical manifestations and prognosis. The aim of this study is to make a breast cancer model suitable for basic, preclinical and clinical studies. Materials and methods: Five virgin Bulb/c mice aged 6 weeks and spontaneous Murine Mammary Adenocarcinoma Cells (MMAC) were used. After anesthesia induction, 1-3 million of MMACs were subcutaneously injected into the mice’s right flank. Four weeks after the tumor induction, tumor size in both length and width were weekly measured using caliper for 4 weeks. Histopathology studies were done 2 months later by Scraff- Boom-Richardson grading. Results: Visible tumors were appeared after 4 weeks. Take-rate of all cells was nearly 100%. Tumor sizes reached 0.6±0.1 mm3, 1.1±0.3, 1.68±0.5 and 3±0.7 cm3 with at fifth, sixth, seventh and eighth weeks respectively. The histopathological studies using H & E method showed invasive ductal carcinoma, grade II/III. Conclusions: Elucidation of the molecular mechanisms of breast cancer progression and metastasis has extremely gained from mouse models in which some stages of tumor progression are recapitulated. https://bccr.tums.ac.ir/index.php/bccrj/article/view/35 https://bccr.tums.ac.ir/index.php/bccrj/article/download/35/pdf