Original Articles

Genistein a complementary cell cycle regulatory effect of Imatinib on Philadelphia positive leukemia cell lines

Abstract

Abstract   This study investigated the possible role of Genistein as a combination with Imatinib in controlling leukemia cell lines proliferation. Three cell lines K562, Kcl22 and CCRF were cultured in in vitro environment and analyzed for MTT, LDH, apoptosis and cycle cell gene expression in the presence of different dosages of Imatinib and Genistein in combination or separately. Data has shown a decrease in proliferation and an increase in apoptosis activity during combination treatment. LDH assay has shown no additional toxicity due to Genistein consumption in combination therapy. Analysis the expression of responsible genes for cell cycle demonstrated that G1 and G2 regulation in combination treatment. Altogether, this study suggests that the combination treatment of Imatinib and Genistein for treating leukemia cells resistant to Imatinib, can increase the efficiency of treatment.
Files
IssueVol 13 No 1 (2021) QRcode
SectionOriginal Articles

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
1.
Azizi E, Biglari A, Abolhassani A, Amanpour S, Muhammadnejad S, Haddadi M, Saffari M, Eslamifar A, Shirkoohi R. Genistein a complementary cell cycle regulatory effect of Imatinib on Philadelphia positive leukemia cell lines. Basic Clin Cancer Res. 2021;13(1):21-29.