Effect of Iron Superoxide and Nickel Oxide Nanoparticles Alone and Combined with Coenzyme Q10 on hsa_circ_0001518 Expression in Breast Tumor-bearing BALB/c Mice
Abstract
Background and Goal:After lung cancer, breast cancer is considered as the second prevailed type of cancer among women. Circular RNAs are a group of non-coding RNAs that through endogenous RNAs’ mechanism play role in tumorigenesis and progression of malignancies. However, little information is known about their role and importance in cancer progression and their chemical resistance. The research has been performed to the aim of studying effect of effective dosages of Iron superoxide and nickel oxide nanoparticles, and Q10 antioxidant alone or simultaneously on hsa_circ_0001518 expression in mice with breast cancer compared to healthy mice. Materials and Methods: In this experimental study, 120 mature female mice BALB/c (Five groups of 10 mice each) have been studied in two groups of healthy mice and those with breast cancer. Inducing breast cancer has been taken place through injection of 4T1 cell line to mice. IC50has been specified on 4T1 cell line through 48 hours treatment with iron superoxide (50, 100, 150, and 200mcg/ml) and nickel oxide (10, 20, 30, and 40mcg/ml) nanoparticles, and Q10 antioxidant (20, 60, 80, and 100mcg/ml). Finally, effect of IC50in treatments alone and combination therapy with iron superoxide, nickel oxide, and Q10 antioxidant on hsa_circ_0001518 has been evaluated, using real-time-PCR. Findings: The results from bioinformatic analysis of circBase showed that hsa_circ_0001518 affects Bcl2 apoptosis inhibitor gene and can play role in creation and progression of breast cancer through anti-apoptotic effects. IC50has been calculated to be respectively equal to 42.92, 49.21, and 47.83mcg/ml for nanoparticles of iron superoxide, nickel oxide, and Q10 antioxidant. The results related for real time PCR showed that expression level of hsa_circ_0001518 under combination therapy with nanoparticles of iron superoxide, nickel oxide and Q10 antioxidant in cancerous cells compared to healthy cells has shown significant reduction. Discussion and Conclusion:The research results confirm increase of cell damage resulted from oxidative stress of nanoparticles of iron superoxide and nickel oxide in combination therapy with Q10 antioxidant compared to treatments alone in cancerous mice. So, using nanoparticles and Q10 simultaneously can be considered in designing a medicine for breast cancer treatment. Oxidative stress resulted from nanoparticle treatment in combination with Q10 can have inhibitory effect on Q10 antioxidant properties in cancerous cells; and, with induction of apoptosis; it may lead to decrease of hsa_circ_0001518 expression. Therefore, studying changes of expression level of hsa_circ_0001518 can be taken into consideration in future and after performance of follow up studies as a molecular biomarker in breast cancer diagnosis and target therapy.
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3. Fang R, Zhu Y, Hu L, Khadka VS, Ai J, Zou H, et al. Plasma Mi¬croRNA Pair Panels as Novel Biomarkers for Detection of Ear¬ly-Stage Breast Cancer. Front Physiol. 2019; 9:1879.
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14. Jadidi Kouhbanani MA, Sadeghipour Y, Sarani M, Sefidgar E, Ilkhani S, Mohammad Amani A, et al. The inhibitory role of syn¬thesized Nickel oxide nanoparticles against Hep-G2, MCF-7, and HT-29 cell lines: the inhibitory role of NiONPs against Hep-G2, MCF-7, and HT-29 cell lines. Green Chemistry Letters and Re¬views. 2021;14:443-453.
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16. Rezaei M, Mafakheri H, Khoshgard K, Montazerabadi A, Mo¬hammadbeigi A, Oubar F. The Cytotoxicity of Dextran-coated Iron Oxide Nanoparticles on Hela and MCF-7 Cancerous Cell Lines. IJT. 2017;5:31-36.
17. Perumal Raj K, Sivakarthik P, Uthirakumar AP, Thangaraj V. Cy¬totoxicity assessment of synthesized nickel oxide nanoparticles on MCF-7 and A-549 cancer cell lines. Journal of Chemical and Pharmaceutical Sciences. 2014:269-271.
18. Greenlee H, Shaw J, Lau YI, Naini A, Maurer M. Lack of effect of coenzyme q10 on doxorubicin cytotoxicity in breast cancer cell cultures. Integr Cancer Ther. 2012;11:243-50.
19. Hu C, Huang Y, Luo P, Yang Y. Effect of antioxidants coenzyme Q10 and β carotene on the cytotoxicity of vemurafenib against hu¬man malignant melanoma. Oncology Letters. 2021;21: 208.
20. Aponte B, Carlos DJ, Hernández J. Role of coenzyme q10 in breast cancer. Pharm Pharmacol Int J. 2015;3:261-267.
21. Wani KD, Kadu BS, Mansara P, Gupta P, Deore AV, Chikate RC, et al. Synthesis, characterization and in vitro study of biocom¬patible cinnamaldehyde functionalized magnetite nanoparticles (CPGF Nps) for hyperthermia and drug delivery applications in breast cancer. PLoS One. 2014;9:107315.
22. Morovati A, Ahmadian S, Jafary H. Cytotoxic effects and apopto¬sis induction of cisplatin-loaded iron oxide nanoparticles modi¬fied with chitosan in human breast cancer cells. Mol Biol Rep. 2019;46:5033-5039.
23. Kavithaa K, Paulpandi M, Padma PR, Sumathi S. Induction of intrinsic apoptotic pathway and cell cycle arrest via baicalein loaded iron oxide nanoparticles as a competent nano-mediated system for triple negative breast cancer therapy. RSC Advances. 2016;6: 64531–64543.
24. Zhou J, Zhang WW, Peng F, Sun JY, He ZY, Wu SG. Downregula¬tion of hsa_circ_0011946 suppresses the migration and invasion of the breast cancer cell line MCF-7 by targeting RFC3. Cancer management and research. 2018;10:535-44.
25. Tang YY, Zhao P, Zou TN, Duan JJ, Zhi R, Yang SY, et al. Cir¬cular RNA hsa_circ_0001982 Promotes Breast Cancer Cell Car¬cinogenesis Through Decreasing miR-143. DNA and cell biology. 2017; 36:901-8.
26. Shi P, Sun J, He B, Song H, Li Z, Kong W, et al. Profiles of dif¬ferentially expressed circRNAs in esophageal and breast cancer. Cancer Manag Res. 2018;10:2207-2221.
27. Zhang HD, Jiang LH, Sun DW, Hou JC, Ji ZL. CircRNA: a novel type of biomarker for cancer. Breast Cancer. 2018;25:1-7.
28. Wang H, Xiao Y, Wu L, Ma D. Comprehensive circular RNA pro¬filing reveals the regulatory role of the circRNA-000911/miR-449a pathway in breast carcinogenesis. Int J Oncol. 2018;52:743-54.
29. Zhao J, Zou H, Han C, Ma J, Zhao J, Tang J. Circlular RNA BARD1 (Hsa_circ_0001098) overexpression in breast cancer cells with TCDD treatment could promote cell apoptosis via miR- 3942/BARD1 axis. Cell Cycle. 2018;17:2731-2744
2. Sharma GN, Dave R, Sanadya J, Sharma P, Sharma KK. Various types and management of breast cancer: an overview. J Adv Pharm Technol Res. 2010;1:109-126.
3. Fang R, Zhu Y, Hu L, Khadka VS, Ai J, Zou H, et al. Plasma Mi¬croRNA Pair Panels as Novel Biomarkers for Detection of Ear¬ly-Stage Breast Cancer. Front Physiol. 2019; 9:1879.
4. Heneghan HM, Miller N, Lowery AJ, Sweeney KJ, Kerin MJ. Mi¬croRNAs as Novel Biomarkers for Breast Cancer. J Oncol. 2009; 2009:950201.
5. Thoidingjam S, Tiku AB. New developments in breast cancer therapy: role of iron oxide nanoparticles. Advances in Natural Sciences: Nanoscience and Nanotechnology. 2017;8: 023002.
6. He X, Xu T, Hu W, Tan Y, Wang D, Wang Y, et al. Circular RNAs: Their Role in the Pathogenesis and Orchestration of Breast Can¬cer. Frontiers in Cell and Developmental Biology. 2021; 9:647736 .
7. Cheng D, Wang J, Dong Z. Xiang L. Cancer-related circular RNA: diverse biological functions. Cancer Cell Int. 2021; 21:11.
8. Xiaozhu T, Hongyan R, Mengjie G, Jinjun Q, Ye Y, Chunyan G. Review on circular RNAs and new insights into their roles in cancer. Computational and Structural Biotechnology Journal. 2021;19: 910-928.
9. Shi P, Sun J, He B, Song H, Li Z, Kong W, et al. Profiles of dif¬ferentially expressed circRNAs in esophageal and breast cancer. Cancer Manag. 2018; 10:2207–2221.
10. Yang Q, Du WW, Wu N, Yang W, Awan FM, Fang L, et al. A cir¬cular RNA promotes tumorigenesis by inducing c-myc nuclear translocation. Cell Death Differ. 2017;24:1609–20.
11. Hafizi M, Soleimani M, Noorian S, Kalanaky S, Fakharzadeh S, Tavakolpoor Saleh N, et al. Effects of BCc1 nanoparticle and its mixture with doxorubicin on survival of murine 4T1 tumor mod¬el. Onco Targets Ther. 2019;12:4691-4701.
12. Razak NA, Abu N, Ho WY, Zamberi MR, Tan SW, Banu N, et al. Cytotoxicity of eupatorin in MCF-7 and MDA-MB-231 human breast cancer cells via cell cycle arrest, anti-angiogenesis and in¬duction of apoptosis. Sci Rep 2019;9:1514.
13. Chi XJ, Wei LL, Bu Q, Mo N, Chen XY, Lan D. et al. Identification of high expression profiles of miR-31-5p and its vital role in lung squamous cell carcinoma: a survey based on qRT-PCR and bioin¬formatics analysis. Transl Cancer Res. 2019;8:788-801.
14. Jadidi Kouhbanani MA, Sadeghipour Y, Sarani M, Sefidgar E, Ilkhani S, Mohammad Amani A, et al. The inhibitory role of syn¬thesized Nickel oxide nanoparticles against Hep-G2, MCF-7, and HT-29 cell lines: the inhibitory role of NiONPs against Hep-G2, MCF-7, and HT-29 cell lines. Green Chemistry Letters and Re¬views. 2021;14:443-453.
15. Hu Z, Yudong H, Shaofan S, Wenchao G, Yuhuan Y, Peiyi T, et al. Visible Light Driven Photodynamic Anticancer Activity of Graphene Oxide/TiO2 Hybrid. Carbon. N. Y. 2012; 50: 994–1004.
16. Rezaei M, Mafakheri H, Khoshgard K, Montazerabadi A, Mo¬hammadbeigi A, Oubar F. The Cytotoxicity of Dextran-coated Iron Oxide Nanoparticles on Hela and MCF-7 Cancerous Cell Lines. IJT. 2017;5:31-36.
17. Perumal Raj K, Sivakarthik P, Uthirakumar AP, Thangaraj V. Cy¬totoxicity assessment of synthesized nickel oxide nanoparticles on MCF-7 and A-549 cancer cell lines. Journal of Chemical and Pharmaceutical Sciences. 2014:269-271.
18. Greenlee H, Shaw J, Lau YI, Naini A, Maurer M. Lack of effect of coenzyme q10 on doxorubicin cytotoxicity in breast cancer cell cultures. Integr Cancer Ther. 2012;11:243-50.
19. Hu C, Huang Y, Luo P, Yang Y. Effect of antioxidants coenzyme Q10 and β carotene on the cytotoxicity of vemurafenib against hu¬man malignant melanoma. Oncology Letters. 2021;21: 208.
20. Aponte B, Carlos DJ, Hernández J. Role of coenzyme q10 in breast cancer. Pharm Pharmacol Int J. 2015;3:261-267.
21. Wani KD, Kadu BS, Mansara P, Gupta P, Deore AV, Chikate RC, et al. Synthesis, characterization and in vitro study of biocom¬patible cinnamaldehyde functionalized magnetite nanoparticles (CPGF Nps) for hyperthermia and drug delivery applications in breast cancer. PLoS One. 2014;9:107315.
22. Morovati A, Ahmadian S, Jafary H. Cytotoxic effects and apopto¬sis induction of cisplatin-loaded iron oxide nanoparticles modi¬fied with chitosan in human breast cancer cells. Mol Biol Rep. 2019;46:5033-5039.
23. Kavithaa K, Paulpandi M, Padma PR, Sumathi S. Induction of intrinsic apoptotic pathway and cell cycle arrest via baicalein loaded iron oxide nanoparticles as a competent nano-mediated system for triple negative breast cancer therapy. RSC Advances. 2016;6: 64531–64543.
24. Zhou J, Zhang WW, Peng F, Sun JY, He ZY, Wu SG. Downregula¬tion of hsa_circ_0011946 suppresses the migration and invasion of the breast cancer cell line MCF-7 by targeting RFC3. Cancer management and research. 2018;10:535-44.
25. Tang YY, Zhao P, Zou TN, Duan JJ, Zhi R, Yang SY, et al. Cir¬cular RNA hsa_circ_0001982 Promotes Breast Cancer Cell Car¬cinogenesis Through Decreasing miR-143. DNA and cell biology. 2017; 36:901-8.
26. Shi P, Sun J, He B, Song H, Li Z, Kong W, et al. Profiles of dif¬ferentially expressed circRNAs in esophageal and breast cancer. Cancer Manag Res. 2018;10:2207-2221.
27. Zhang HD, Jiang LH, Sun DW, Hou JC, Ji ZL. CircRNA: a novel type of biomarker for cancer. Breast Cancer. 2018;25:1-7.
28. Wang H, Xiao Y, Wu L, Ma D. Comprehensive circular RNA pro¬filing reveals the regulatory role of the circRNA-000911/miR-449a pathway in breast carcinogenesis. Int J Oncol. 2018;52:743-54.
29. Zhao J, Zou H, Han C, Ma J, Zhao J, Tang J. Circlular RNA BARD1 (Hsa_circ_0001098) overexpression in breast cancer cells with TCDD treatment could promote cell apoptosis via miR- 3942/BARD1 axis. Cell Cycle. 2018;17:2731-2744
| Files | ||
| Issue | Vol 13 No 2 (2021) | |
| Section | Original Articles | |
| DOI | https://doi.org/10.18502/bccr.v13i2.10027 | |
| Keywords | ||
| breast cancer iron superoxide nanoparticles nickel oxide nanoparticles Q10 antioxidant circRNA | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Ganjipour G, Heshmati M, Hashemi M, Entezari M. Effect of Iron Superoxide and Nickel Oxide Nanoparticles Alone and Combined with Coenzyme Q10 on hsa_circ_0001518 Expression in Breast Tumor-bearing BALB/c Mice. Basic Clin Cancer Res. 2022;13(2):84-91.
