Effect of Quercetin and Intermittent and Continuous Exercise on Catalase, Superoxide dismutase, and Malondialdehyde in the Heart of Rats with Colon Cancer
Abstract
Background: Colorectal cancer is the fourth leading cause of death globally, and the second most common cancer in Europe. About 8% of all cancer-related deaths occur due to colorectal cancer, and the highest prevalence has been reported in Asia and Eastern Europe. Methods: In this experimental study, 80 rats were divided into two groups of cases (n=70) and controls (n=10). Colorectal cancer was induced weekly in rats by subcutaneous injection of 15 mg/kg Azoxymethane. The rats were then divided into 7 experimental subgroups of patients, saline, quercetin, intermittent exercise, continuous exercise, quercetin plus intermittent, and quercetin plus continuous exercise. Oxidative stress biomarkers, including superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) were measured in the rats' heart tissue by the ELISA method. Data were analyzed using ANOVA by SPSS software. Results: Oxidative stress in heart cells increased due to colorectal cancer. Quercetin alone or in combination with exercise significantly increased mean levels of CAT and SOD in the heart tissue of rats compared with patient and saline groups (P<0.0001). In contrast, the MDA level was significantly decreased (P<0.05). Conclusion: Colorectal cancer increased the oxidative stress in cardiac cells. Quercetin alone improved oxidative stress in cardiac tissue, and its combination with exercise was more effective.
1. Nunez‐Sanchez MA, González‐Sarrias A, Romo‐Vaquero M, García‐Villalba R, Selma MV, Tomás‐Barberán FA, et al. Dietary phenolics against colorectal cancer—From promising preclinical results to poor translation into clinical trials: Pitfalls and future needs. Molecular nutrition & food research. 2015;59(7):91-1274
2. Thrift AP. The epidemic of oesophageal carcinoma: Where are we now? Cancer epidemiology. 2016;95-41;88
3. Cai H, Scott E, Kholghi A, Andreadi C, Rufini A, Karmokar A, et al. Cancer chemoprevention: Evidence of a nonlinear dose response for the protective effects of resveratrol in humans and mice. Science translational medicine. 2015;7(298):298
4. Boughey JC, Hartmann LC, Anderson SS, Degnim AC, Vierkant RA, Reynolds CA, et al. Evaluation of the Tyrer-Cuzick (International Breast Cancer Intervention Study) model for breast cancer risk prediction in women with atypical hyperplasia. Journal of Clinical Oncology. 2010;28(22):6-3591
5. Alizadeh AM, Khaniki M, Azizian S, Mohaghgheghi MA, Sadeghizadeh M, Najafi F. Chemoprevention of azoxymethane-initiated colon cancer in rat by using a novel polymeric nanocarrier–curcumin. European journal of pharmacology. 2012;689(1):32-226
6. Siddiqui IA, Adhami VM, Ahmad N, Mukhtar H. Nanochemoprevention: sustained release of bioactive food components for cancer prevention. Nutr Cancer. 2010;67(7):90-883.
7. Shukla Y, Singh R. Resveratrol and cellular mechanisms of cancer prevention. Annals of the New York Academy of Sciences. 2011;1215(1):1-8
8. Inoue H, Kishimoto A, Ushikoshi-Nakayama R, Hasaka A, Takahashi A, Ryo K, et al. Resveratrol improves salivary dysfunction in a non-obese diabetic (NOD) mouse model of Sjogren's syndrome. Journal of clinical biochemistry and nutrition. 2016;59(2):12-107
9. Park HG, Lee YR, Jun JK, Lee WL. Exercise training is more effective than resveratrol supplementation on alleviation of inflammation in peritoneal macrophages of high fat diet mice. Journal of exercise nutrition & biochemistry. 2014;18(1):79-87
10. Hajighasem A, Farzanegi P, Mazaheri Z. Effects of combined therapy with resveratrol, continuous and interval exercises on apoptosis, oxidative stress, and inflammatory biomarkers in the liver of old rats with non-alcoholic fatty liver disease. Archives of Physiology and Biochemistry 2018;in perss:ttps://doi.org/10.1080/13813455.2018.1441872.
11. Moon YJ, Wang X, Morris ME. Dietary flavonoids: effects on xenobiotic and carcinogen metabolism. Toxicology in vitro. 2006;20(2):187-210
12. van der Woude H, ter Veld MG, Jacobs N, van der Saag PT, Murk AJ, Rietjens IM. The stimulation of cell proliferation by quercetin is mediated by the estrogen receptor. Molecular nutrition & food research. 2005;49(8):71-763
13. Granado-Serrano AB, Martín MA, Bravo L, Goya L, Ramos S. Quercetin induces apoptosis via caspase activation, regulation of Bcl-2, and inhibition of PI-3-kinase/Akt and ERK pathways in a human hepatoma cell line (HepG2). The Journal of nutrition. 2006;136(11):215-271.
14. Cho S-Y, Park S-J, Kwon M-J, Jeong T-S, Bok S-H, Choi W-Y, et al. Quercetin suppresses proinflammatory cytokines production through MAP kinases and NF-κB pathway in lipopolysaccharide-stimulated macrophage. Molecular and cellular biochemistry. 2003;243(1-2):153-60
15. Guo R, Liong E, So K, Fung M, Tipoe G. Beneficial mechanisms of aerobic exercise on hepatic lipid metabolism in non-alcoholic fatty liver disease. Hepatobiliary Pancreat Dis Int. 2015;139-14-44
16. Batacan RB, Jr., Duncan MJ, Dalbo VJ, Connolly KJ, Fenning AS. Light-intensity and high-intensity interval training improve cardiometabolic health in rats. Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme. 2016;41(9):945-52
17. Freitas DA, Rocha-Vieira E, Soares BA, Nonato LF, Fonseca SR, Martins JB, et al. High intensity interval training modulates hippocampal oxidative stress, BDNF and inflammatory mediators in rats. Physiology & behavior. 2017;11-184
18. Gonzalez-Rodriguez A, Mayoral R, Agra N, Valdecantos MP, Pardo V, Miquilena-Colina ME, et al. Impaired autophagic flux is associated with increased endoplasmic reticulum stress during the development of NAFLD. Cell death & disease. 2014;5-11
19. Thong-Ngam D, Samuhasaneeto S, Kulaputana O, Klaikeaw N. N-acetylcysteine attenuates oxidative stress and liver pathology in rats with non-alcoholic steatohepatitis. World journal of gastroenterology. 2007;13(38):5127-32
20. Schneider Y, Duranton B, Goss F, Schleiffer R, Seiler N, Raul F. Resveratrol inhibits intestinal tumorigenesis and modulates host-defense-related gene expression in an animal model of human familial adenomatous polyposis. Nutrition and cancer. 2001;39(1):102-7
21. Volate SR, Davenport DM, Muga SJ, Wargovich MJ. Modulation of aberrant crypt foci and apoptosis by dietary herbal supplements (quercetin, curcumin, silymarin, ginseng and rutin). Carcinogenesis. 2005;26(8):1450-6
22. Wang D, Li SP, Fu JS, Bai L, Guo L. Resveratrol augments therapeutic efficiency of mouse bone marrow mesenchymal stem cell-based therapy in experimental autoimmune encephalomyelitis. International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience. 2016;6-6-49
23. Chen Q, Wang T, Li J, Wang S, Qiu F, Yu H, et al. Effects of Natural Products on Fructose-Induced Nonalcoholic Fatty Liver Disease (NAFLD). Nutrients. 2017;92.
24. Wang ZM, Chen YC, Wang DP. Resveratrol, a natural antioxidant, protects monosodium iodoacetate-induced osteoarthritic pain in rats. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2016;763;70-83
25. Sun S, Zhang M, Yang Q, Shen Z, Chen J, Yu B, et al. Resveratrol suppresses lipoprotein-associated phospholipase A2 expression by reducing oxidative stress in macrophages and animal models. Molecular nutrition & food research. 2017;63-70
26. Elbe H, Gul M, Cetin A, Taslidere E, Ozyalin F, Turkoz Y, et al. Resveratrol reduces light and electron microscopic changes in acetaminophen-induced hepatotoxicity in rats: Role of iNOS expression. Ultrastructural pathology. 2017;10-20
27. Faghihzadeh F, Adibi P, Rafiei R, Hekmatdoost A. Resveratrol supplementation improves inflammatory biomarkers in patients with nonalcoholic fatty liver disease. Nutrition research. 2014;34(10):837-43
2. Thrift AP. The epidemic of oesophageal carcinoma: Where are we now? Cancer epidemiology. 2016;95-41;88
3. Cai H, Scott E, Kholghi A, Andreadi C, Rufini A, Karmokar A, et al. Cancer chemoprevention: Evidence of a nonlinear dose response for the protective effects of resveratrol in humans and mice. Science translational medicine. 2015;7(298):298
4. Boughey JC, Hartmann LC, Anderson SS, Degnim AC, Vierkant RA, Reynolds CA, et al. Evaluation of the Tyrer-Cuzick (International Breast Cancer Intervention Study) model for breast cancer risk prediction in women with atypical hyperplasia. Journal of Clinical Oncology. 2010;28(22):6-3591
5. Alizadeh AM, Khaniki M, Azizian S, Mohaghgheghi MA, Sadeghizadeh M, Najafi F. Chemoprevention of azoxymethane-initiated colon cancer in rat by using a novel polymeric nanocarrier–curcumin. European journal of pharmacology. 2012;689(1):32-226
6. Siddiqui IA, Adhami VM, Ahmad N, Mukhtar H. Nanochemoprevention: sustained release of bioactive food components for cancer prevention. Nutr Cancer. 2010;67(7):90-883.
7. Shukla Y, Singh R. Resveratrol and cellular mechanisms of cancer prevention. Annals of the New York Academy of Sciences. 2011;1215(1):1-8
8. Inoue H, Kishimoto A, Ushikoshi-Nakayama R, Hasaka A, Takahashi A, Ryo K, et al. Resveratrol improves salivary dysfunction in a non-obese diabetic (NOD) mouse model of Sjogren's syndrome. Journal of clinical biochemistry and nutrition. 2016;59(2):12-107
9. Park HG, Lee YR, Jun JK, Lee WL. Exercise training is more effective than resveratrol supplementation on alleviation of inflammation in peritoneal macrophages of high fat diet mice. Journal of exercise nutrition & biochemistry. 2014;18(1):79-87
10. Hajighasem A, Farzanegi P, Mazaheri Z. Effects of combined therapy with resveratrol, continuous and interval exercises on apoptosis, oxidative stress, and inflammatory biomarkers in the liver of old rats with non-alcoholic fatty liver disease. Archives of Physiology and Biochemistry 2018;in perss:ttps://doi.org/10.1080/13813455.2018.1441872.
11. Moon YJ, Wang X, Morris ME. Dietary flavonoids: effects on xenobiotic and carcinogen metabolism. Toxicology in vitro. 2006;20(2):187-210
12. van der Woude H, ter Veld MG, Jacobs N, van der Saag PT, Murk AJ, Rietjens IM. The stimulation of cell proliferation by quercetin is mediated by the estrogen receptor. Molecular nutrition & food research. 2005;49(8):71-763
13. Granado-Serrano AB, Martín MA, Bravo L, Goya L, Ramos S. Quercetin induces apoptosis via caspase activation, regulation of Bcl-2, and inhibition of PI-3-kinase/Akt and ERK pathways in a human hepatoma cell line (HepG2). The Journal of nutrition. 2006;136(11):215-271.
14. Cho S-Y, Park S-J, Kwon M-J, Jeong T-S, Bok S-H, Choi W-Y, et al. Quercetin suppresses proinflammatory cytokines production through MAP kinases and NF-κB pathway in lipopolysaccharide-stimulated macrophage. Molecular and cellular biochemistry. 2003;243(1-2):153-60
15. Guo R, Liong E, So K, Fung M, Tipoe G. Beneficial mechanisms of aerobic exercise on hepatic lipid metabolism in non-alcoholic fatty liver disease. Hepatobiliary Pancreat Dis Int. 2015;139-14-44
16. Batacan RB, Jr., Duncan MJ, Dalbo VJ, Connolly KJ, Fenning AS. Light-intensity and high-intensity interval training improve cardiometabolic health in rats. Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme. 2016;41(9):945-52
17. Freitas DA, Rocha-Vieira E, Soares BA, Nonato LF, Fonseca SR, Martins JB, et al. High intensity interval training modulates hippocampal oxidative stress, BDNF and inflammatory mediators in rats. Physiology & behavior. 2017;11-184
18. Gonzalez-Rodriguez A, Mayoral R, Agra N, Valdecantos MP, Pardo V, Miquilena-Colina ME, et al. Impaired autophagic flux is associated with increased endoplasmic reticulum stress during the development of NAFLD. Cell death & disease. 2014;5-11
19. Thong-Ngam D, Samuhasaneeto S, Kulaputana O, Klaikeaw N. N-acetylcysteine attenuates oxidative stress and liver pathology in rats with non-alcoholic steatohepatitis. World journal of gastroenterology. 2007;13(38):5127-32
20. Schneider Y, Duranton B, Goss F, Schleiffer R, Seiler N, Raul F. Resveratrol inhibits intestinal tumorigenesis and modulates host-defense-related gene expression in an animal model of human familial adenomatous polyposis. Nutrition and cancer. 2001;39(1):102-7
21. Volate SR, Davenport DM, Muga SJ, Wargovich MJ. Modulation of aberrant crypt foci and apoptosis by dietary herbal supplements (quercetin, curcumin, silymarin, ginseng and rutin). Carcinogenesis. 2005;26(8):1450-6
22. Wang D, Li SP, Fu JS, Bai L, Guo L. Resveratrol augments therapeutic efficiency of mouse bone marrow mesenchymal stem cell-based therapy in experimental autoimmune encephalomyelitis. International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience. 2016;6-6-49
23. Chen Q, Wang T, Li J, Wang S, Qiu F, Yu H, et al. Effects of Natural Products on Fructose-Induced Nonalcoholic Fatty Liver Disease (NAFLD). Nutrients. 2017;92.
24. Wang ZM, Chen YC, Wang DP. Resveratrol, a natural antioxidant, protects monosodium iodoacetate-induced osteoarthritic pain in rats. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2016;763;70-83
25. Sun S, Zhang M, Yang Q, Shen Z, Chen J, Yu B, et al. Resveratrol suppresses lipoprotein-associated phospholipase A2 expression by reducing oxidative stress in macrophages and animal models. Molecular nutrition & food research. 2017;63-70
26. Elbe H, Gul M, Cetin A, Taslidere E, Ozyalin F, Turkoz Y, et al. Resveratrol reduces light and electron microscopic changes in acetaminophen-induced hepatotoxicity in rats: Role of iNOS expression. Ultrastructural pathology. 2017;10-20
27. Faghihzadeh F, Adibi P, Rafiei R, Hekmatdoost A. Resveratrol supplementation improves inflammatory biomarkers in patients with nonalcoholic fatty liver disease. Nutrition research. 2014;34(10):837-43
| Files | ||
| Issue | Vol 12 No 1 (2020) | |
| Section | Original Articles | |
| DOI | https://doi.org/10.18502/bccr.v12i1.5733 | |
| Keywords | ||
| Oxidative stress Colorectal cancer Intermittent and continuous exercise Quercetin | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Talaei B, Panji M, Nazari Robati F, Tezerji S. Effect of Quercetin and Intermittent and Continuous Exercise on Catalase, Superoxide dismutase, and Malondialdehyde in the Heart of Rats with Colon Cancer. Basic Clin Cancer Res. 2020;12(1):34-41.
