Expression of miR-1290 and Its Target Genes THBS1 and DKK3 in Colorectal Cancer Patients
-
Sima Nobari
,
Mohammad Hasan Soheilifar
,
Hoda Keshmiri Neghab
,
Farid Azizi Jalilian
,
Fatemeh Bahreini
,
Razieh Amini
Abstract
Background: MicroRNAs (miRNAs) are small noncoding RNAs (containing approximately 22 nucleotides), which modulate and control the expression of target genes by binding them. MiRNAs play a crucial role in tumorigenesis. Thus, alterations in the expression level of miRNAs play a key role in the pathobiology of numerous cancers. In this research, the expression level of MicroRNA-1290 (miR1290) and its target genes THBS1 and DKK3 were evaluated in colorectal cancer (CRC) patients.Methods: This case-control study was carried out on 144 paraffin-embedded tissue samples of CRC and adjacent tissues from patients who referred to Imam Khomeini Hospital, Tehran, Iran. Total RNA was isolated from the tissue using Trizol reagent following the manufacturer’s instructions and then reverse transcribed to cDNA.The expression of miR-1290 and its target genes was measured by quantitative Real-Time PCR (qRT-PCR). Statistical analyses were performed using SPSS V.20 statistical software. Results: We present evidence that the miR-1290 expression in CRC tissues was significantly higher than in the normal margin, and its targets were downregulated in tumor tissue compared to the adjacent tissue.Conclusion: This study supports the essential role of miR-1290 and its contribution to CRC invasion and metastasis through targeting THBS1 and DKK3, as biomarkers for CRC diagnosis.
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the reprogramming of colon cancer cells. Cancer Lett.
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Jiang YM, Yu DL, Hou GX, Jiang JL, Zhou Q, Xu XF. Serum thrombospondin-2 is a candidate diagnosis biomarker for early
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Safari E, Mosayebi G, Khorram S. Dkk-3 as a potential biomarker for diagnosis and prognosis of colorectal cancer. Med
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Dolatkhah R, Somi MH, Kermani IA, Ghojazadeh M, Jafarabadi MA, Farassati F, et al. Increased colorectal cancer incidence
in Iran: a systematic review and meta-analysis. BMC Public Health. 2015;15:997.
Azizi H, Asadollahi K, Davtalab Esmaeili E, Mirzapoor M.
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Wang J, Chen J, Sen S. MicroRNA as Biomarkers and Diagnostics.
J Cell Physiol. 2016;231(1):25-30.
Lech G, Slotwinski R, Slodkowski M, Krasnodebski IW. Colorectal cancer tumour markers and biomarkers:
Recent therapeutic advances. World J Gastroenterol.
2016;22(5):1745-55.
Riedmaier I, Pfaffl MW. Transcriptional biomarkers--high throughput screening, quantitative verification, and bioinformatical validation methods. Methods. 2013;59(1):3-9.
Liu X, Xu X, Pan B, He B, Chen X, Zeng K, et al. Circulating miR-1290 and miR-320d as Novel Diagnostic Biomarkers of
Human Colorectal Cancer. J Cancer. 2019;10(1):43-50.
Ma Q, Wang Y, Zhang H, Wang F. miR-1290 Contributes to Colorectal Cancer Cell Proliferation by Targeting INPP4B.
Oncol Res. 2018;26(8):1167-74.
Liu X, Xu X, Pan B, He B, Chen X, Zeng K, et al. Circulating
miR-1290 and miR-320d as Novel Diagnostic Biomarkers of
Human Colorectal Cancer. J Cancer. 2019;10(1):43-50.
Wu J, Ji X, Zhu L, Jiang Q, Wen Z, Xu S, et al. Up-regulation
of microRNA-1290 impairs cytokinesis and affects the reprogramming of colon cancer cells. Cancer Lett.
2013;329(2):155-63.
Park JM, Kim MK, Chi KC, Kim JH, Lee SH, Lee EJ. Aberrant loss of dickkopf-3 in gastric cancer: can it predict lymph node metastasis preoperatively? World J Surg. 2015;39(4):1018-25.
Sargiannidou I, Zhou J, Tuszynski GP. The role of thrombospondin- 1 in tumor progression. Exp Biol Med (Maywood). 2001;226(8):726-33.
Levin B, Lieberman DA, McFarland B, Smith RA, Brooks D, Andrews KS, et al. Screening and surveillance for the early
detection of colorectal cancer and adenomatous polyps, 2008: a joint guideline from the American Cancer Society,
the US Multi-Society Task Force on Colorectal Cancer, and the American College of Radiology. CA Cancer J Clin.
2008;58(3):130-60.
Kim DH, Pickhardt PJ, Taylor AJ, Leung WK, Winter TC, Hinshaw JL, et al. CT colonography versus colonoscopy
for the detection of advanced neoplasia. N Engl J Med. 2007;357(14):1403-12.
Bader AG, Brown D, Stoudemire J, Lammers P. Developing therapeutic microRNAs for cancer. Gene therapy. 2011
Dec;18(12):1121-6.
Robins H, Li Y, Padgett RW. Incorporating structure to predict microRNA targets. Proc Natl Acad Sci U S A.
2005;102(11):4006-9.
Stahlhut C, Slack FJ. MicroRNAs and the cancer phenotype: profiling, signatures and clinical implications. Genome Med.
2013;5(12):111.
Lu J, Getz G, Miska EA, Alvarez-Saavedra E, Lamb J, Peck D, et al. MicroRNA expression profiles classify human cancers.
Nature. 2005;435(7043):834-8.
Waldman SA, Terzic A. MicroRNA signatures as diagnostic and therapeutic targets. Clin Chem. 2008;54(6):943-4.
Ahmed FE, Jeffries CD, Vos PW, Flake G, Nuovo GJ, Sinar
DR, et al. Diagnostic microRNA markers for screening sporadic human colon cancer and active ulcerative colitis in stool
and tissue. Cancer Genomics Proteomics. 2009;6(5):281-95.
Liu J, Li H, Sun L, Shen S, Zhou Q, Yuan Y, et al. Epigenetic
Alternations of MicroRNAs and DNA Methylation Contribute
to Liver Metastasis of Colorectal Cancer. Dig Dis Sci.
2019;64(6):1523-34.
Imaoka H, Toiyama Y, Fujikawa H, Hiro J, Saigusa S, Tanaka K, et al. Circulating microRNA-1290 as a novel diagnostic
and prognostic biomarker in human colorectal cancer. Ann Oncol. 2016;27(10):1879-86.
Wu J, Ji X, Zhu L, Jiang Q, Wen Z, Xu S, et al. Up-regulation of microRNA-1290 impairs cytokinesis and affects
the reprogramming of colon cancer cells. Cancer Lett.
2013;329(2):155-63.
Jiang YM, Yu DL, Hou GX, Jiang JL, Zhou Q, Xu XF. Serum thrombospondin-2 is a candidate diagnosis biomarker for early
non-small-cell lung cancer. Biosci Rep. 2019;39(7).
Safari E, Mosayebi G, Khorram S. Dkk-3 as a potential biomarker for diagnosis and prognosis of colorectal cancer. Med
J Islam Repub Iran. 2018;32:86.
Dolatkhah R, Somi MH, Kermani IA, Ghojazadeh M, Jafarabadi MA, Farassati F, et al. Increased colorectal cancer incidence
in Iran: a systematic review and meta-analysis. BMC Public Health. 2015;15:997.
Azizi H, Asadollahi K, Davtalab Esmaeili E, Mirzapoor M.
Iranian dietary patterns and risk of colorectal cancer. Health Promot Perspect. 2015;5(1):72-80.
Wang J, Chen J, Sen S. MicroRNA as Biomarkers and Diagnostics.
J Cell Physiol. 2016;231(1):25-30.
Lech G, Slotwinski R, Slodkowski M, Krasnodebski IW. Colorectal cancer tumour markers and biomarkers:
Recent therapeutic advances. World J Gastroenterol.
2016;22(5):1745-55.
Riedmaier I, Pfaffl MW. Transcriptional biomarkers--high throughput screening, quantitative verification, and bioinformatical validation methods. Methods. 2013;59(1):3-9.
Liu X, Xu X, Pan B, He B, Chen X, Zeng K, et al. Circulating miR-1290 and miR-320d as Novel Diagnostic Biomarkers of
Human Colorectal Cancer. J Cancer. 2019;10(1):43-50.
Ma Q, Wang Y, Zhang H, Wang F. miR-1290 Contributes to Colorectal Cancer Cell Proliferation by Targeting INPP4B.
Oncol Res. 2018;26(8):1167-74.
Liu X, Xu X, Pan B, He B, Chen X, Zeng K, et al. Circulating
miR-1290 and miR-320d as Novel Diagnostic Biomarkers of
Human Colorectal Cancer. J Cancer. 2019;10(1):43-50.
Wu J, Ji X, Zhu L, Jiang Q, Wen Z, Xu S, et al. Up-regulation
of microRNA-1290 impairs cytokinesis and affects the reprogramming of colon cancer cells. Cancer Lett.
2013;329(2):155-63.
Park JM, Kim MK, Chi KC, Kim JH, Lee SH, Lee EJ. Aberrant loss of dickkopf-3 in gastric cancer: can it predict lymph node metastasis preoperatively? World J Surg. 2015;39(4):1018-25.
Sargiannidou I, Zhou J, Tuszynski GP. The role of thrombospondin- 1 in tumor progression. Exp Biol Med (Maywood). 2001;226(8):726-33.
| Files | ||
| Issue | Vol 12 No 1 (2020) | |
| Section | Original Articles | |
| DOI | https://doi.org/10.18502/bccr.v12i1.5729 | |
| Keywords | ||
| Colorectal Cancer MiR-1290 THBS1 DKK3 | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Nobari S, Soheilifar MH, Keshmiri Neghab H, Azizi Jalilian F, Bahreini F, Amini R. Expression of miR-1290 and Its Target Genes THBS1 and DKK3 in Colorectal Cancer Patients. Basic Clin Cancer Res. 2020;12(1):10-17.
