Association of Hypoxia-inducible factor α subunits with TSGA10 transcripts in HeLa, MCF7 and MDA-MB231- cell lines
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Parya Rahmani Rad
,
Maryam Beigom Mobasheri
,
Morteza Hashemzadeh-Chaleshtori
,
Mohammad Hossein Modarressi
Abstract
Background: Hypoxia is a common phenomenon in cancer cells, related to angiogenesis and cell proliferation the hypoxia-inducible factor family (HIFs) is the primary transcriptional factor to hypoxic stress. Cancer-testis (CT) antigens are almost expressed in male germ cells, aberrantly expresses in some malignancies as well. The CT gene, TSGA10, prevents the nuclear accumulation of HIF-α and may be involved in organ-specific regulation of hypoxic gene expression during sperm maturation. TSGA10 is supposed to regulate the HIF expression in germ cells and cancer cells. The HIF-α subunit has three isoforms, involved in oxygen transport, angiogenesis and tumor metastasis, which their detection is the subject of the current study.Methods: Three cell lines, MCF7, MDA-MB-231 and HeLa were cultured, passaged and categorized into normal and synchronized groups. The cells were subjected to RNA extraction and reverse-transcribed into cDNAs. Real time RT-PCR was performed to amplify TSGA10 and HIF-α isoforms and HPRT, as the normalizer gene, using appropriate primers. The REST and SPSS software were used for statistical analysis. Results: The expression of three isoforms of HIF-α in HeLa cell line was higher than MCF7, and MCF7 was higher than MDA-MB-231. Moreover, the expression relationship between HIF-α isoforms and TSGA10 was evaluated in each three cell lines as well. The results were significant in all cases with P =0.01. Before and after synchronization in each three cell lines, the isoform expressions of HIF-α and TASGA10 were evaluated, and the results were revealed their dependent expression. The relationship between HIF-α isoforms and TSGA10 expression was compared with each other. The cell lines with less TSGA10 expression had the higher expression of HIF-α isoforms and vice versa, according to the extent of TSGA10.Conclusion: The significant relationship between expressions of TSGA10 and HIF-α isoforms is confirmed.Modarressi MH, Cameron J, Taylor KE, Wolfe J. Identification and characterization of a novel gene, TSGA10, expressed in testis. Gene. 2001; 262(1-2):249-55.
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| Files | ||
| Issue | Vol 9 No 3 (2017) | |
| Section | Original Articles | |
| Keywords | ||
| TSGA10 HIF-α subunits Cancer-Testis genes breast cancer cell lines MCF7 MDA-MB-231 | ||
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