Long Stress-induced Non-coding Transcript 5 rs2935641 Polymorphism and the risk of Bladder Cancer in Patients from Fars Province
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Abstract
Background: Bladder cancer is one of the most common cancers in the world with considerably higher frequency among men than women. Treatment of the disease is possible according to early diagnosis, so identifying useful markers is very important. LSINCT5 (long stress-induced non-coding transcript 5) is a stress-regulated lncRNA involved in response to oxidative stress, also in cell proliferation and cancer development, especially in the presence of tobacco carcinogens. in this study, we aimed to evaluate the influence of LSINCT5 gene polymorphism with the susceptibility of bladder cancer among a group of patients from south of Iran.Methods: 100 patients with bladder cancer and 100 healthy subjects as control group were investigated in this case-control study.Genotyping of the rs2935641 polymorphism was carried out using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) approach. Results: It was found that the single base substitution C to T at the coding region of the LSINCT5 was not significantly associated with bladder cancer. Genotype and allele frequencies distributed evenly among cases and controls, but no TT genotype was seen. History of smoking was significantly higher in bladder cancer patients compared to controls (p<0.001). Conclusion: Results showed that the LSINCT5 polymorphism was not a key factor in the pathogenesis of bladder cancer. Tobacco smoking is one of the strongest risk factors for bladder cancer.Yamada T, Tsuchiya K, Kato S, Kamei S, Taniguchi M,Takeuchi T, Yamamoto N, Ehara H, Deguchi T. A pretreat- ment nomogram predicting recurrence- and progression-free survival for nonmuscle invasive bladder cancer in Japanese patients. Int J Clin Oncol. 2010; 15:271-9.
Ahmadi M, Ranjbaran H, Momeninejad AM, Nozari J,Mirzajani MR, Azadbakht M, Hosseinimehr SJ. Epide- miologic and Socioeconomic Status of Bladder Cancer in Mazandaran province Northern Iran. Asian Pacific J Cancer Prev. 2012; 13(10):5053-5056.
Sexton WJ, Wiegand LR, Correa JJ, Politis C, IravaniDickinson S, Kang LC. Bladder Cancer: A Review of Non–Muscle-Invasive Disease. Cancer Control. 2010; 17(4):256-68.
Otunctemur A, Koklu I, Ozbek E, Dursun M, Sahin S,Besiroglu H, Erkoc M, Danis E, Bozkurt M, Gurbuz A.Are Bladder Neoplasms More Aggressive in Patients with a Smoking-related Second Malignancy? Asian Pac J Cancer Prev. 2014; 15:4025-8.
Chung KT. The Etiology of Bladder Cancer and its Pre-vention. J Cancer Sci Ther. 2013; 5:346-361.
Fajkovic H, Halpern A, Cha K, Bahadori A, Chromecki TF, Karakiewicz RI, Breinl E, Merseburger AS, Shariat SF.Impact of gender on bladder cancer incidence, staging, and prognosis. Word J Urol. 2011; 29:457-463.
Ghafouri-Fard S, Nekoohesh L, Motevaseli E. Bladder Cancer Biomarkers: Review and Update. Asian Pac J Cancer Prev. 2014; 15(6):2395-2403.
Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell 2011; 144:646-674.
Zhang Q, Su M, Lu G, Wang J. The complexity of bladder cancer: long noncoding RNA are on the stage. Mol Cancer BioMed Central. 2013; 12:101.
Bhartiya D, Pa K, Ghosh S, Kapoor S, Jalali S, Panwar B.lncRNome: a comprehensive knowledgebase of human long noncoding RNAs. Database (Oxford). 2013; 2013:34.
Martens-Uzunova ES, Bottcher R, Croce CM, Jenster G,Visakorpi T, Calin GA. Long non-coding RNA in the pros-tate, bladder, and kidney cancer. Eur Urol. 2014; 65: 1140-1151.
Zhang H, Chen Z, Wang X, Huang Z, He Z, Chen Y.Long non-coding RNA: a new player in cancer. J Hematol Oncol. 2013; 6:37.
Ye F, Wang L, Castillo-Martin M, McBride R, Galsky M D, Zhu J, Boffetta P, Zhang DY, Cordon-Cardo C. Biomark- ers for bladder cancer management: present and future. Am J Clin Exp Urol. 2014; 2(1):1-14.
Silva JM, Boczek NJ, Berres MW, Ma X, Smith DI. LSINCT5 is overexpressed in breast and ovarian cancer and affects cellular proliferation. RNA Biol. 2011; 8(3):496-505.
Silva JM, Perez DS, Pritchett JR, Halling ML, Tang H, Smith DI. Identification of long stress-induced non-coding transcripts that have altered expression in cancer. Genomics.2010; 95:355-362.
Anonymous exploring the biological contributions to hu-man health: does sex matter? J Womens Health Gend Based Med. 2001; 10:433–9.
Wein AJ, Kavoussi LR, Novick AC, Partin AW, Peters CA. Urothelial tumors of the bladder. Campbell-Walsh Urol-ogy. 2008.
Salehi A, Khezri A, Malekmakan L, Aminsharifi A. Epi-demiologic Status of Bladder Cancer in Shiraz, Southern Iran.Asian Pacific J Cancer Prev. 2011; 12:1323-1327.
Farahmand M, Khademolhosseini F, Medhati M, Shokr-pour N, Joulaei H, Mehrabani D. Trend of Bladder Cancer inFars Province, Southern Iran. Iran Red Crescent Med J. 2009;11(4):2002-2006.
Aminsharifi AR, Ghaderi A, Naeimi S, Khezri AZ, Aga- hi N, Aminian A, Nima Zamiri. CTLA-4 Exon One +49A/G Gene Variants in Patients with Superficial and Invasive Blad- der Cancer: A Study in Southern Iran. Middle East j Cancer.2010; 1(1):15-20.
Paz-y-Miño C, Muñoz MJ, López-Cortés A, Cabrera A,Palacios A, Castro B, Paz-y-Miño N, Sánchez ME. Frequen-cy of Polymorphisms pro198leu in GPX-1 Gene and ile58thr in MnSOD Gene in the Altitude Ecuadorian PopulationWith Bladder Cancer. Oncol Res. 2010; 18(8):395–400.
Chung KT. The etiology of bladder cancer and its preven-tion. J Cancer Sci Ther. 2013; 5(10):346-361.
Scélo G, Brennan P. The epidemiology of bladder and kidney cancer. Nat Clin Pract Urol. 2007; 4:205–217.
Sawicka E, Lisowska A, Kowal P, Dlugosz A. The role of oxidative stress in bladder cancer. Postepy Hig Med Dosw.2015; 69:744-52.
Murata M, Tamura A, Tada M, Kawanishi S. Mechanism of oxidative DNA damage induced by carcinogenic 4-amino-biphenyl. Free Radic Biol Med. 2001; 30:765-773.
Spartz L, Bloom AD. Biological consequences of ox- idative stress: Implications for cardiovascular disease and carcinogenesis. 1sted. New York: Oxford University Press.1992:138-61.
Friedberg EC, Meira LB. Database of mouse strains carry-ing targeted mutations in genes affecting biological responses
to DNA damage version 7. DNA Repair. 2006; 5:189-209.
Ploeg M, Aben KK, Kiemeney LA. The present and future burden of urinary bladder cancer in the world. World J Urol.2009; 27:289-293.
Siriwardhana N, Choudhary S, Wang HC. Precancerous model of human breast epithelial cells induced by NNK for prevention. Breast Cancer Treat. 2008; 109(3):427-41.
Hoffmann RA, Hecht SS. The biological significance of tobacco-specific N-nitrosamines: smoking and adenocarcino- ma of the lung. Crit Rev Toxicol. 1996; 26:199–211.
Xu MD, Qi P, Weng WW, Shen XH, Ni SJ, Dong L, Huang D, Tan C, Sheng WQ, Zhou XY, Du X. Long non-cod- ing RNA LSINCT5 predicts negative prognosis and exhibits oncogenic activity in gastric cancer. Medicine (Baltimore).2014; 93(28):e303.
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| Issue | Vol 8 No 1&2 (2016) | |
| Section | Original Articles | |
| Keywords | ||
| Bladder cancer LSINCT5 LncRNA Polymorphism | ||
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