Assessment of in vitro chromosomal sensitivity to low doses of gamma irradiation in patients with acute lymphoblastic leukemia
-
Asghar Ramyar
,
Asghar Aghamohammadi
,
Hossein Mozdarani
,
Majid Mahmoodi
,
Cyrus Azimi
,
Zahra Safari
,
Hassan-Ali Nedaei
,
Fareedeh Farzanfar
,
Nima Rezaei
,
Majid Asadi-Shekaari
,
Mahbod Esfahani
,
Seyed Kazem Hosseini
,
Mahdi Yazdani
,
Hassan Abolhassani
,
Fatemeh Khalesi
,
Mohamad Ali Mohagheghi
Abstract
Background: Acute lymphoblastic leukemia (ALL) constitutes a heterogeneous group of diseases characterized by abnormal proliferation and accumulation of immature lymphoblasts arrested at various differentiation stages. Increasing evidence suggests that chromosomal defects are present in these patients. The aim of this study is to investigate the chromosomal radiosensitivity in a group of ALL patients.Methods: To analyze chromosomal radiosensitivity of ALL patients, lymphocytes of 20 patients were cultured followed by exposure to γ-ray irradiation to detect the chromosomal aberrations as an indicator of radiosensitivity. Cells were scored for the number of aberrations (chromatid breaks, chromatid gaps, chromosome breaks, chromosome gaps and chromatid exchanges). Results were compared with healthy individuals, and ataxia telangiectasia (AT) patients as positive control.Results: On average number of aberrations in ALL patients was significantly higher than that in healthy controls.  We found 65% of ALL patients appeared to be susceptible to in vitro irradiation. Chromosomal radiosensitivity of 35% patients was not different from healthy donors. Ataxia telangiectasia patients showed the highest degree of lymphocyte radiosensitivity. The results also indicate that there is a good correlation between the two assays, G2 and G0, using the same blood sample for both assays.Conclusion: According to the result, we concluded that most of the ALL patients are sensitive to ionizing radiation and therefore should be protected from unnecessary diagnostic and therapeutic procedures using ionizing radiation.Czuczman MS, Dodge RK, Stewart CC, Frankel SR, Davey FR, Powell BL, et al. Value of immunophenotype in intensively treated adult acute lymphoblastic leukemia: cancer and leukemia Group B study 8364. Blood. 1999 Jun 1;93(11):3931-9.
Bruchova H, Kalinova M, Brdicka R. Array-based analysis of gene expression in childhood acute lymphoblastic leukemia. Leuk Res. 2004 Jan;28(1):1-7.
Uckun FM, Sensel MG, Sun L, Steinherz PG, Trigg ME, Heerema NA, et al. Biology and treatment of childhood T-lineage acute lympho- blastic leukemia. Blood. 1998 Feb 1;91(3):735-46.
Ahmad F, Dalvi R, Chavan D, Das BR, Mandava S. Cytogenetic pro- file of acute lymphocytic leukemia patients: report of a novel transloca- tion t(4;13) (q21 x 3; q35) from an Indian population. Hematology. 2008 Feb;13(1):28-33.
Chiaretti S, Foa R. T-cell acute lymphoblastic leukemia. Haemato- logica. 2009 Feb;94(2):160-2.
Greaves MF. Aetiology of acute leukaemia. Lancet. 1997 Feb 1;349(9048):344-9.
Cytogenetic abnormalities in adult acute lymphoblastic leukemia: correlations with hematologic findings outcome. A Collaborative Study of the Group Francais de Cytogenetique Hematologique. Blood. 1996 Apr 15;87(8):3135-42.
Ferrando AA, Look AT. Clinical implications of recurring chromo- somal and associated molecular abnormalities in acute lymphoblastic leukemia. Semin Hematol. 2000 Oct;37(4):381-95.
Riches AC, Bryant PE, Steel CM, Gleig A, Robertson AJ, Preece PE, et al. Chromosomal radiosensitivity in G2-phase lymphocytes identifies breast cancer patients with distinctive tumour characteristics. Br J Can- cer. 2001 Oct 19;85(8):1157-61.
Distel LV, Neubauer S, Keller U, Sprung CN, Sauer R, Grabenbauer GG. Individual differences in chromosomal aberrations after in vitro irradiation of cells from healthy individuals, cancer and cancer suscep- tibility syndrome patients. Radiother Oncol. 2006 Dec;81(3):257-63.
Scott D, Barber JB, Levine EL, Burrill W, Roberts SA. Radiation- induced micronucleus induction in lymphocytes identifies a high fre- quency of radiosensitive cases among breast cancer patients: a test for predisposition? Br J Cancer. 1998 Feb;77(4):614-20.
Papworth R, Slevin N, Roberts SA, Scott D. Sensitivity to radiation- induced chromosome damage may be a marker of genetic predisposi- tion in young head and neck cancer patients. Br J Cancer. 2001 Mar 23;84(6):776-82.
Mozdarani H, Mansouri Z, Haeri SA. Cytogenetic radiosensitivity of g0-lymphocytes of breast and esophageal cancer patients as determined by micronucleus assay. J Radiat Res (Tokyo). 2005 Mar;46(1):111-6.
Palanduz S, Palanduz A, Yalcin I, Somer A, Ones U, Ustek D, et al. In vitro chromosomal radiosensitivity in common variable immune deficiency. Clin Immunol Immunopathol. 1998 Feb;86(2):180-2.
Gatti RA, Boder E, Good RA. Immunodeficiency, radiosensitivity, and the XCIND syndrome. Immunol Res. 2007;38(1-3):87-101.
Aghamohammadi A, Moin M, Kouhi A, Mohagheghi MA, Shirazi A, Rezaei N, et al. Chromosomal radiosensitivity in patients with com- mon variable immunodeficiency. Immunobiology. 2008;213(5):447-54.
Tucker SL, Geara FB, Peters LJ, Brock WA. How much could the radiotherapy dose be altered for individual patients based on a predic- tive assay of normal-tissue radiosensitivity? Radiother Oncol. 1996 Feb;38(2):103-13.
Uckun FM, Sather H, Gaynon P, Arthur D, Nachman J, Sensel M, et al. Prognostic significance of the CD10+CD19+CD34+ B-progeni- tor immunophenotype in children with acute lymphoblastic leukemia: a report from the Children’s Cancer Group. Leuk Lymphoma. 1997 Nov;27(5-6):445-57.
Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DA, Gral- nick HR, et al. Proposals for the classification of the acute leukaemias. French-American-British (FAB) co-operative group. Br J Haematol. 1976 Aug;33(4):451-8.
Bene MC, Castoldi G, Knapp W, Ludwig WD, Matutes E, Orfao A, et al. Proposals for the immunological classification of acute leukemias. European Group for the Immunological Characterization of Leukemias (EGIL). Leukemia. 1995 Oct;9(10):1783-6.
Scott D, Barber JB, Spreadborough AR, Burrill W, Roberts SA. In- creased chromosomal radiosensitivity in breast cancer patients: a com- parison of two assays. Int J Radiat Biol. 1999 Jan;75(1):1-10.
Baccichet A, Qualman SK, Sinnett D. Allelic loss in childhood acute lymphoblastic leukemia. Leuk Res. 1997 Sep;21(9):817-23.
Pui CH, Robison LL, Look AT. Acute lymphoblastic leukaemia. Lancet. 2008 Mar 22;371(9617):1030-43.
Felix CA. Secondary leukemias induced by topoisomerase-targeted drugs. Biochim Biophys Acta. 1998 Oct 1;1400(1-3):233-55.
Liang DC, Yang CP, Lin DT, Hung IJ, Lin KH, Chen JS, et al. Long-term results of Taiwan Pediatric Oncology Group studies 1997 and 2002 for childhood acute lymphoblastic leukemia. Leukemia. 2010 Feb;24(2):397-405.
Vrooman LM, Neuberg DS, Stevenson KE, Asselin BL, Athale UH, Clavell L, et al. The low incidence of secondary acute myelogenous leu- kaemia in children and adolescents treated with dexrazoxane for acute lymphoblastic leukaemia: a report from the Dana-Farber Cancer Insti- tute ALL Consortium. Eur J Cancer. 2011 Jun;47(9):1373-9.
Dann EJ, Rowe JM. Biology and therapy of secondary leukaemias. Best Pract Res Clin Haematol. 2001 Mar;14(1):119-37.
Bonassi S, Hagmar L, Stromberg U, Montagud AH, Tinnerberg H, Forni A, et al. Chromosomal aberrations in lymphocytes predict hu- man cancer independently of exposure to carcinogens. European Study Group on Cytogenetic Biomarkers and Health. Cancer Res. 2000 Mar 15;60(6):1619-25.
Hagmar L, Stromberg U, Bonassi S, Hansteen IL, Knudsen LE, Lindholm C, et al. Impact of types of lymphocyte chromosomal aber- rations on human cancer risk: results from Nordic and Italian cohorts. Cancer Res. 2004 Mar 15;64(6):2258-63.
Sun X, Becker-Catania SG, Chun HH, Hwang MJ, Huo Y, Wang Z, et al. Early diagnosis of ataxia-telangiectasia using radiosensitivity testing. J Pediatr. 2002 Jun;140(6):724-31.
Baria K, Warren C, Roberts SA, West CM, Scott D. Chromosomal radiosensitivity as a marker of predisposition to common cancers? Br J Cancer. 2001 Apr 6;84(7):892-6.
Howe OL, Daly PA, Seymour C, Ormiston W, Nolan C, Mother- sill C. Elevated G2 chromosomal radiosensitivity in Irish breast can- cer patients: a comparison with other studies. Int J Radiat Biol. 2005 May;81(5):373-8.
De Ruyck K, de Gelder V, Van Eijkeren M, Boterberg T, De Neve W, Vral A, et al. Chromosomal radiosensitivity in head and neck cancer patients: evidence for genetic predisposition? Br J Cancer. 2008 May 20;98(10):1723-38.
Baeyens A, Thierens H, Claes K, Poppe B, Messiaen L, De Ridder L, et al. Chromosomal radiosensitivity in breast cancer patients with a known or putative genetic predisposition. Br J Cancer. 2002 Dec 2;87(12):1379-85.
| Files | ||
| Issue | Vol 3 No 2 (2011) | |
| Section | Original Articles | |
| Keywords | ||
| Chromosomal sensitivity gamma irradiation acute lympho blastic leukemia | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
