Methylation Profile of Von Hipple Lindau Gene Promoter in Acute Lymphoblastic Leukemia Patients
AbstractBackground: Epigenetic alteration, with DNA methylation being the major mechanism, can influence gene expression without alteringDNAsequence, which may lead to cancer development. Tumor suppressor genes such as VHL (von Hippel-Lindau) when affected by epigenetic alteration will be silenced. This study investigated DNA methylation of VHL gene in ALL (Acute Lymphoblastic Leukemia) patients with methylation specific PCR (MSP).Methods: DNA of 26 ALL patients and 26 healthy control subject were extracted, treated with bisulfite, and subsequently their VHL gene methylation was analyzed by MSP technique.Results: None of the patients were methylated for VHL gene.Conclusions: Due to the lack of methylation in VHL gene promoter in patients and healthy group, the methylation of this gene cannot be used in diagnosis and prognosis of ALL patients.Keywords: Precursor Cell Lymphoblastic Leukemia-Lymphoma, von Hippel-Lindau Disease, DNA Methylation, Epigenomics.
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