Tehran University of Medical Sciences Basic & Clinical Cancer Research 2228-6527 13 3 2022 05 18 156 174 Elham Bahrami Salehloo National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Najme Mozdoori Estahban higher education center, Department of cellular and molecular biology, Shiraz, Iran Mohammad javad Dehghan Esmatabadi Malek - Ashtar University of Technology, Tehran, Iran Samira Hajigholami Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Ali Bozorgmehr Iran Psychiatric Hospital, Iran University of Medical Sciences (IUMS), Tehran, Iran 2021 08 27 2021 12 06 Progress in cancer stem cells has opened up a new window to develop better cancer treatment methods. Several pre-clinical and clinical trial studies use CSCs targeting via surface markers method and inhibition of stem cell pathway to eradicate cancer. Albite, some important question was unclear about CSCs origin and molecular mechanism of self-renewal, the structure of CSCs markers and so on, but the eradication of these cells eliminate cancer. In this review, we have argued about the CSC surface markers on different cancers, the mechanisms of action, and therapeutic procedures related to the cancer biomarkers. Then, we have discussed the challenges of these therapies. https://bccr.tums.ac.ir/index.php/bccrj/article/view/398