Tehran University of Medical Sciences Basic & Clinical Cancer Research 2228-6527 7 2&3 2015 11 02 20 31 EN Majid Motovali-Bashi Genetics Division, Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran Zahra Sadeghi Genetics Division, Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran Simin Hemati Department of Radiotherapy and Oncology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 2015 11 28 2015 11 28 Aim: MMP enzymes are a family of membrane proteins that are capable of digesting extracellular matrix compounds (ECM) and basement membrane. Matrilysin enzyme is the smallest member of MMP family that is encoded by MMP-7 gene (matrilysin). According to the reports, G allele of -181 A/G single nucleotide polymorphism of MMP-7 gene causes an increase in the expression of this enzyme. This study aims to  investigate the effect of promoter single nucleotide polymorphism of this gene on the colorectal cancer and compare  the accuracy of tetra-primer ARMS PCR technique using RFLP-PCR technique. Methods: In this study, genomic DNA was extracted from total blood of 61 patients with colorectal cancer and 77 healthy subjects as controls. They were genotyped by tetra-primer ARMS PCR technique. A number of randomly selected samples were later genotyped by RFLP-PCR technique. Results: : As expected, the results of RFLP-PCR technique confirmed the results of tetra-primer ARMS technique.  Results of this study showed no statistical association between this polymorphism and risk of initiation and metastasis of the colorectal cancer in the population of Isfahan. Conclusion: ATetra-primer ARMS technique can be used as an efficient clinical technique. https://bccr.tums.ac.ir/index.php/bccrj/article/view/174 https://bccr.tums.ac.ir/index.php/bccrj/article/download/174/pdf