Tehran University of Medical Sciences Basic & Clinical Cancer Research 2228-6527 7 1 2015 10 19 16 23 EN Zohreh Abdolmaleki Department of pharmacology, Faculty of Veterinary Medicine University, Tehran, Iran Hossein-Ali Arab Department of pharmacology, Faculty of Veterinary Medicine University, Tehran, Iran Saeid Amanpour Tumor Model Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Farrokh Tirgari Cancer Research Center, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran Kamran Mansouri Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran Samad Muhammadnejad Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran. 2015 10 17 2015 10 17 Background: Artemisinin and its derivatives are well known as anti-malaria drugs and particularly useful for the treatment of infection of Plasmodium falciparum malaria parasites. In recent years, there are many reports of anticancer activities of artemisinins both in vitro and in vivo. This study aimed to investigate the cytotoxicity and anti-angiogenic activity of the ethanolic extract of Artemisia sieberi (EEA) in comparison to its active substance, artemisinin (ART). Methods: The cytotoxic effects of EEA and ART on the HCT-116 colorectal adenocarcinoma cells line were studied by clonogenic assay.The inhibitory effect of test compounds on angiogenesis was evaluated by three-dimensional culture cells model on human umbilical vein endothelial cells (HUVECs) in cytodex-3-microcarriers as in vitro model. Results: The results showed that EEA significantly started inhibition of colony-forming capacity of HCT-116 cells line at concentrations of 1 up to 100µg/ml (P < 0.001). We also found EEA and ART markedly reduced the angiogenic activities on HUVECs culture in a concentration-dependent fashion (P < 0.001, r = 0.997 and P< 0.001, r = 0.998 respectively). Conclusion: It seems that EEA has high cytotoxic effect on HCT-116 cells line and also it is a potent inhibitor of angiogenesis in cultured cells. These results provide the primary findings for further investigations on therapeutic or preventive properties of Artemisia sieberi on cancer. https://bccr.tums.ac.ir/index.php/bccrj/article/view/165 https://bccr.tums.ac.ir/index.php/bccrj/article/download/165/01