Tehran University of Medical Sciences Basic & Clinical Cancer Research 2228-6527 4 3&4 2012 10 01 23 33 EN Samaneh Hajihoseiny Division of Genetics, Department of Biology, Facult of Sciences, University of Isfahan, Iran Majid Motovali-Bashi Division of Genetics, Department of Biology, Facult of Sciences, University of Isfahan, Iran Mahnaz Roaei Department of Radiotherapy and Oncology, Faculty of Medicine, Isfahan University of Medical Sciences, Iran. 2014 04 24 2014 09 28 Single nucleotide polymorphism is the most frequent type of polymorphism in the genome sequence, mostly used as the genetic marker for identification. Moreover,  point mutations are diagnosable and detectable similar to single nucleotide polymorphisms which cause genetic diseases. Thus, it is important to suggest a technique to detect many mutations with high speed and accuracy and low cost. Tetra-primer  ARMS PCR is able to provide the possibility of identifying different allele forms of a single nucleotide polymorphism in a PCR reaction. In this study, two common point mutations of IVSII-I (G-A) and IVSI-110(G-A) in beta thalassemia were detected and genotyped by tetra-primer ARMS technique. Finally, accuracy and precision of this techniques were analyzed and evaluated using sequencing results. As the results of genotyping by tetra-primer ARMS and sequencing were the same, it seems that the former is capable to turn into a routine test in diagnostic laboratories and clinics. https://bccr.tums.ac.ir/index.php/bccrj/article/view/128 https://bccr.tums.ac.ir/index.php/bccrj/article/download/128/001