Nanohybrid Platform of Functionalized Graphene Oxide for Chemo-Photothermal Therapy

  • Mohadeseh Hashemi 1- Faculty of new Science and Engineering, The University of Tehran, Tehran 1439957131, Iran.
  • Meisam Omidi Protein Research Centre, Shahid Beheshti University, GC, Velenjak, Tehran 1985717443, Iran.
  • Javad Mohammadi Faculty of new Science and Engineering, The University of Tehran, Tehran 1439957131, Iran.
  • Mohammad Shalbaf Protein Research Centre, Shahid Beheshti University, GC, Velenjak, Tehran 1985717443, Iran.
  • Javad Shabani Shayeh Protein Research Centre, Shahid Beheshti University, GC, Velenjak, Tehran 1985717443, Iran.
  • Mohammad Ali Mohagheghi Tehran University of Medical Sciences, Cancer Research Center, Tehran, 1419733141, Iran.
Keywords: Herceptin, ploy (L-lysine), graphene oxide

Abstract

Background: Despite the enormous effort has been done for cancer therapy, fabricating targeted drug delivery platform which can effectively eliminate cancer is a challenge.Methods: In this study, we have developed a novel platform composed of graphene oxide (GO), poly-l-lysine (PLL), Herceptin (Her) and doxorubicin (DOX) for chemo-photothermal therapy. GO has been prepared using the hummers method. The morphology of the prepared carriers has studied using transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The successful conjugation of PLL and Her to the surface of GO has been examined using Fourier-transform infrared spectroscopy (FTIR). DOX loading on GO sheets was characterized using UV-Vis absorption spectra. MTT and live/dead assay have been administrated to study the synergistic chemo-photothermal therapy potential of the carries. Results: FTIR shows the successful conjugation of the PLL and Herceptin to the GO surface. TGA analysis suggests that, in comparison to GO, GO-PLL has higher thermal stability. In addition, DOX loading efficiency is around 78.5 ± 4.3 %. Also, Live /dead and MTT assays reveal that the introduced carrier can effectively kill cancerous cells via chemo-photothermal effects. Conclusion: Our results have suggested that the novel carrier is a versatile platform for chemo-photothermal therapy application.

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Published
2018-08-14
How to Cite
1.
Hashemi M, Omidi M, Mohammadi J, Shalbaf M, Shabani Shayeh J, Mohagheghi MA. Nanohybrid Platform of Functionalized Graphene Oxide for Chemo-Photothermal Therapy. BCCR. 10(4):1-.